Journal
/
Biochemistry
/
顺磁弛豫增强，用于检测和表征固有无序蛋白质的自缔合
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
Please note that all translations are automatically generated. Click here for the English version.

顺磁弛豫增强，用于检测和表征固有无序蛋白质的自缔合

English

Automatically Generated

1,497 Views

•

07:24 min

•

September 23, 2021

DOI:

10.3791/63057-v

DOI:
10.3791/63057-v

•

07:24 min
September 23, 2021

•

8 Views
,
1Department of Biochemistry and Structural Biology and Greehey Children’s Cancer Research Institute,The University of Texas Health Science Center at San Antonio

Summary

Automatically generated

提出了一种应用顺磁弛豫增强核磁共振波谱来检测固有无序蛋白质中弱和瞬态分子间和分子内相互作用的方案。

Explore More Videos

Paramagnetic Relaxation EnhancementIntrinsically Disordered ProteinsBiomolecular InteractionsNMR TechniquesSpin Label15N Isotopically Labeled ProteinGel FiltrationEllman’s ReagentNMR Sample PreparationPulse Calibration

Read Article

Cite this Article

Johnson, C. N., Libich, D. S. Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins. J. Vis. Exp. (175), e63057, doi:10.3791/63057 (2021).

Download .ris file

Copy

Share Video

.

Copy