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Mikroakışkan Cihaz Kullanılarak siRNA Yüklü Lipid Nanopartiküllerinin Üretimi
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device
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Mikroakışkan Cihaz Kullanılarak siRNA Yüklü Lipid Nanopartiküllerinin Üretimi

Türkçe

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7,677 Views

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06:02 min

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March 22, 2022

DOI:

10.3791/62999-v

DOI:
10.3791/62999-v

•

06:02 min
March 22, 2022

•

48 Views
, , , , , ,
1Division of Applied Chemistry, Faculty of Engineering,Hokkaido University, 2JST PRESTO, 3Graduate School of Chemical Sciences and Engineering,Hokkaido University

Summary

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RNA teslimatı da dahil olmak üzere ilaç dağıtım sistemlerinde (DDS) mikroakışkan bazlı lipid nanopartikül (LNP) üretim yöntemleri dikkat çekmiştir. Bu protokol, iLiNP adlı orijinal mikroakışkan cihazımızı kullanarak imalat, LNP (siRNA yüklü LNP) üretimi ve LNP değerlendirme süreçlerini açıklar.

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Cite this Article

Maeki, M., Okada, Y., Uno, S., Niwa, A., Ishida, A., Tani, H., Tokeshi, M. Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device. J. Vis. Exp. (181), e62999, doi:10.3791/62999 (2022).

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