Journal
/
Bioquímica
/
Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device
JoVE Revista
Bioquímica
This content is Free Access.
JoVE Revista Bioquímica
Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device

Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device

7,575 Views

•

06:02 min

•

March 22, 2022

DOI:

10.3791/62999-v

DOI:
10.3791/62999-v

•

06:02 min
March 22, 2022

•

48 Views
, , , , , ,
1Division of Applied Chemistry, Faculty of Engineering,Hokkaido University, 2JST PRESTO, 3Graduate School of Chemical Sciences and Engineering,Hokkaido University

Summary

Automatically generated

Microfluidic-based lipid nanoparticle (LNP) production methods have attracted attention in drug delivery systems (DDSs), including RNA delivery. This protocol describes the fabrication, LNP (siRNA-loaded LNP) production, and LNP evaluation processes using our original microfluidic device named iLiNP.

Explore More Videos

SiRNALipid NanoparticlesMicrofluidic DeviceSize ControlBiodistributionGene SilencingMRNARibonucleoproteinDOTAPDSPCCholesterolDMG-PEG2kSalineAcetate BufferSiGL4Dynamic Light ScatteringFlow RateFlow Rate Ratio

Read Article

Cite this Article

Maeki, M., Okada, Y., Uno, S., Niwa, A., Ishida, A., Tani, H., Tokeshi, M. Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device. J. Vis. Exp. (181), e62999, doi:10.3791/62999 (2022).

Download .ris file

Copy

Share Video

.

Copy