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Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device

Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device

DOI:
10.3791/62999-v

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06:02 min

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March 22, 2022

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, , , , , ,
1Division of Applied Chemistry, Faculty of Engineering,Hokkaido University, 2JST PRESTO, 3Graduate School of Chemical Sciences and Engineering,Hokkaido University

Bölümler

  • 00:04Introduction
  • 00:54Preparation of Lipid Solutions and the siRNA/ Buffer Solution
  • 01:58Set-Up of the iLiNP Device and Production of LNPs
  • 02:35Dialysis of the LNP Suspension and LNP Size Measurement
  • 03:24Results: POPC LNP Size Distributions Produced by the iLiNP Device at Different Flow Rate Ratios and Characterization and Cytotoxicity of siRNA-Loaded LNPs
  • 05:22Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Microfluidic-based lipid nanoparticle (LNP) production methods have attracted attention in drug delivery systems (DDSs), including RNA delivery. This protocol describes the fabrication, LNP (siRNA-loaded LNP) production, and LNP evaluation processes using our original microfluidic device named iLiNP.

Etiketler

SiRNALipid NanoparticlesMicrofluidic DeviceSize ControlBiodistributionGene SilencingMRNARibonucleoproteinDOTAPDSPCCholesterolDMG-PEG2kSalineAcetate BufferSiGL4Dynamic Light ScatteringFlow RateFlow Rate Ratio

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