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Journal / Biochemistry / Production of siRNA-Loaded Lipid Nanoparticles…
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Biochemistry

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JoVE Journal Biochemistry
Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device
 

Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device

Article DOI: 10.3791/62999-v • 06:02 min • March 22nd, 2022
March 22nd, 2022 •
1,2, 3, 3, 3, 1, 1, 1
1Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, 2JST PRESTO, 3Graduate School of Chemical Sciences and Engineering, Hokkaido University

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Microfluidic-based lipid nanoparticle (LNP) production methods have attracted attention in drug delivery systems (DDSs), including RNA delivery. This protocol describes the fabrication, LNP (siRNA-loaded LNP) production, and LNP evaluation processes using our original microfluidic device named iLiNP.

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SiRNA-loaded Lipid Nanoparticles Microfluidic Device LNP Size Control Method Nano Medicines RNA Delivery Systems ILiNP Device DOTAP DSPC Cholesterol DMG-PEG2k Solutions Lipid Ethanol Solutions SiRNA Buffer Solution Lipid And Aqueous Solutions Syringe Pumps LNP Suspensions
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