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Interrogatório de Mecanobiologia totalmente óptica de proteína associada a sim em câncer humano e células normais usando um sistema multifuncional
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09:55 min
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December 20, 2021
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1Department of Electrical and Computer Engineering, Herbert Wertheim College of Engineering,University of Florida, 2Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering,University of Florida, 3Department of Electrical and Computer Engineering,University of California, 4Department of Radiation Oncology, College of Medicine,University of Florida, 5UF Health Cancer Center,University of Florida, 6Department of Physics, College of Liberal Arts and Sciences,University of Florida, 7Department of Anatomy and Cell Biology, College of Medicine,University of Florida
Summary
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Este artigo apresenta um protocolo passo a passo detalhado sobre como utilizar um sistema multifuncional e programável integrado ao usuário que permite que a imagem multicanal e a análise mecanobiológica automática e a elucidação da mecano-sensibilidade da proteína associada ao Sim (YAP).
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Cite this Article
Luo, Q., Huang, M., Liang, C., Zhang, J., Lin, G., Yu, S., Tanaka, M., Lepler, S., Guan, J., Siemann, D., Tang, X. All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System. J. Vis. Exp. (178), e62934, doi:10.3791/62934 (2021).
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