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All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System
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December 20, 2021
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1Department of Electrical and Computer Engineering, Herbert Wertheim College of Engineering,University of Florida, 2Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering,University of Florida, 3Department of Electrical and Computer Engineering,University of California, 4Department of Radiation Oncology, College of Medicine,University of Florida, 5UF Health Cancer Center,University of Florida, 6Department of Physics, College of Liberal Arts and Sciences,University of Florida, 7Department of Anatomy and Cell Biology, College of Medicine,University of Florida
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This paper presents a detailed stepwise protocol on how to utilize an integrated multi-functional and user-programmable system that enables automatic multi-channel imaging and mechanobiological analysis to elucidate the mechano-sensitivity of Yes-associated protein (YAP).
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MechanobiologyYes-associated ProteinCancer CellsNormal CellsMulti-functional SystemOptical ElectrophysiologySuper-resolution ImagingLive-cell ImagingHigh-throughputSelf-drift CorrectingLong-term ImagingFluorescent MicroscopyNYCOMEnvironment ChamberMotorized StageCO2 FlowTemperature ControlGlass-bottom Petri DishConfocal ControllerMicroManagerAdhesive TapeAdhesive GlueIntelliJAMFIP
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Luo, Q., Huang, M., Liang, C., Zhang, J., Lin, G., Yu, S., Tanaka, M., Lepler, S., Guan, J., Siemann, D., Tang, X. All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System. J. Vis. Exp. (178), e62934, doi:10.3791/62934 (2021).
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