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Interrogation mécanobiologique entièrement optique de protéines associées au Oui dans le cancer humain et les cellules normales à l’aide d’un système multifonctionnel
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December 20, 2021
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1Department of Electrical and Computer Engineering, Herbert Wertheim College of Engineering,University of Florida, 2Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering,University of Florida, 3Department of Electrical and Computer Engineering,University of California, 4Department of Radiation Oncology, College of Medicine,University of Florida, 5UF Health Cancer Center,University of Florida, 6Department of Physics, College of Liberal Arts and Sciences,University of Florida, 7Department of Anatomy and Cell Biology, College of Medicine,University of Florida
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Cet article présente un protocole détaillé par étapes sur la façon d’utiliser un système intégré multifonctionnel et programmable par l’utilisateur qui permet l’imagerie multicanal automatique et l’analyse mécanobiologique pour élucider la mécanosensibilité de la protéine associée au Oui (YAP).
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Cite this Article
Luo, Q., Huang, M., Liang, C., Zhang, J., Lin, G., Yu, S., Tanaka, M., Lepler, S., Guan, J., Siemann, D., Tang, X. All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System. J. Vis. Exp. (178), e62934, doi:10.3791/62934 (2021).
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