This paper presents a detailed stepwise protocol on how to utilize an integrated multi-functional and user-programmable system that enables automatic multi-channel imaging and mechanobiological analysis to elucidate the mechano-sensitivity of Yes-associated protein (YAP).
Luo, Q., Huang, M., Liang, C., Zhang, J., Lin, G., Yu, S., Tanaka, M., Lepler, S., Guan, J., Siemann, D., Tang, X. All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System. J. Vis. Exp. (178), e62934, doi:10.3791/62934 (2021).