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原位 斑马鱼幼体和幼鱼在雌雄同体发育过程中的杂交
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development
DOI:

08:16 min

August 13, 2021

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Chapters

  • 00:04Introduction
  • 00:32Raising Larvae
  • 01:03Days 1-2: Fixing Larvae and Day 3: Measuring Larvae
  • 02:16Days 3 4: Dehydration and Day 5: Rehydration, Proteinase K Digestion, Bleaching and Prehybridization
  • 04:05Day 6: Probe Hybridization and Day 7: Probe Washing and Blocking
  • 05:38Days 8 9: Antibody Incubation and Days 10 11: Antibody Washing
  • 06:05Day 12: Staining and Imaging
  • 07:18Results: In Situ Hybridization for Gene Expression Analysis in Zebrafish Larvae and Juveniles During Mesonephros Development
  • 07:52Conclusion

Summary

Automatic Translation

斑马鱼是了解肾脏发育的重要模型。在这里, 对原位 杂交方案进行了优化,以检测斑马鱼幼虫和幼鱼在中胚层发育过程中的基因表达。

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