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In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development
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发育生物学
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JoVE 杂志 发育生物学
In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development

In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development

2,341 Views

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08:16 min

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August 13, 2021

DOI:

10.3791/62930-v

DOI:
10.3791/62930-v

•

08:16 min
August 13, 2021

•

10 Views
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1Department of Biology,Indiana University of Pennsylvania

Summary

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The zebrafish is an important model for understanding kidney development. Here, an in situ hybridization protocol is optimized to detect gene expression in zebrafish larvae and juveniles during mesonephros development.

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In Situ HybridizationZebrafish LarvaeZebrafish JuvenilesMesonephros DevelopmentGene ExpressionProbe PenetrationKidney VisualizationMatingEmbryo CollectionLarva FixationLarva DehydrationLarva RehydrationProteinase K DigestionBleaching

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Cite this Article

Kasar, S. N., Grandinette, S. A., Semelsberger, S. D., Diep, C. Q. In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development. J. Vis. Exp. (174), e62930, doi:10.3791/62930 (2021).

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