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果蝇
成腿的解剖和免疫组织化学,以检测已识别运动神经元的神经肌肉接头处的变化
JoVE Journal
Neuroscience
Author Produced
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JoVE Journal
Neuroscience
Dissection and Immunohistochemistry of the
Drosophila
Adult Leg to Detect Changes at the Neuromuscular Junction for an Identified Motor Neuron
Please note that all translations are automatically generated.
Click here for the English version.
果蝇
成腿的解剖和免疫组织化学,以检测已识别运动神经元的神经肌肉接头处的变化
DOI:
10.3791/62844-v
•
11:02 min
•
February 12, 2022
•
Geoff Stilwell
,
Anthony Agudelo*
1,2
1
Department of Biology
,
Rhode Island College
,
2
Department of Pediatrics, Women and Infants Hospital
,
Warren Alpert Medical School of Brown University
Chapters
00:01
Introduction
01:23
Initial Fixation of Tissues
03:13
Removing Leg Cuticle and Antibody Staining
06:58
Mounting Legs
07:41
Results
10:37
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一种解剖技术,该技术保留了神经肌肉接头的结构,并能够对成年
果蝇
腿中的运动神经元进行详细的免疫细胞化学研究。
Tags
Drosophila
Adult Leg
Dissection
Immunohistochemistry
Neuromuscular Junction
Motor Neuron
Neuronal Architecture
Muscle Architecture
Degenerative Changes
Genotypes
Disease Models
Antibodies
Methanol
PBS
Fixation
Forceps
Vannas Scissors
Silicone Elastomer
24-well Plate
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