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Dissection of the Transversus Abdominis Muscle for Whole-mount Neuromuscular Junction Analysis
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Neuroscience
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JoVE Journal Neuroscience
Dissection of the Transversus Abdominis Muscle for Whole-mount Neuromuscular Junction Analysis

Dissection of the Transversus Abdominis Muscle for Whole-mount Neuromuscular Junction Analysis

English

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11,204 Views

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06:12 min

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January 11, 2014

DOI:

10.3791/51162-v

DOI:
10.3791/51162-v

•

06:12 min
January 11, 2014

•

15 Views
, ,
1Regenerative Medicine Program,Ottawa Hospital Research Institute, 2Centre for Integrative Physiology,University of Edinburgh

Summary

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In this video we demonstrate a protocol for dissection of the transversus abdominis muscle of the mouse and use immunofluorescence and microscopy to visualize neuromuscular junctions.

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Transversus AbdominisNeuromuscular JunctionMotor NeuronDissectionImmunofluorescenceSpinal Muscular AtrophyMotor UnitAxonal BranchingTerminal SproutingInnervation Pattern

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Murray, L., Gillingwater, T. H., Kothary, R. Dissection of the Transversus Abdominis Muscle for Whole-mount Neuromuscular Junction Analysis. J. Vis. Exp. (83), e51162, doi:10.3791/51162 (2014).

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