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Dissection and Immunohistochemistry of the Drosophila Adult Leg to Detect Changes at the Neuromuscular Junction for an Identified Motor Neuron
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Dissection and Immunohistochemistry of the Drosophila Adult Leg to Detect Changes at the Neuromuscular Junction for an Identified Motor Neuron

Dissection and Immunohistochemistry of the Drosophila Adult Leg to Detect Changes at the Neuromuscular Junction for an Identified Motor Neuron

3,866 Views

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11:02 min

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February 12, 2022

DOI:

10.3791/62844-v

DOI:
10.3791/62844-v

•

11:02 min
February 12, 2022

•

11 Views
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1Department of Biology,Rhode Island College, 2Department of Pediatrics, Women and Infants Hospital,Warren Alpert Medical School of Brown University

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We describe a dissection technique that preserves the architecture of the neuromuscular junction and enables a detailed immunocytochemical study of motor neurons in the adult Drosophila leg.

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DrosophilaAdult LegDissectionImmunohistochemistryNeuromuscular JunctionMotor NeuronNeuronal ArchitectureMuscle ArchitectureDegenerative ChangesGenotypesDisease ModelsAntibodiesMethanolPBSFixationForcepsVannas ScissorsSilicone Elastomer24-well PlateFA SolutionNutator

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Stilwell, G., Agudelo, A. Dissection and Immunohistochemistry of the Drosophila Adult Leg to Detect Changes at the Neuromuscular Junction for an Identified Motor Neuron. J. Vis. Exp. (180), e62844, doi:10.3791/62844 (2022).

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