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Isolatie en in vitro kweek van beenmerg-afgeleide macrofagen voor de studie van NO-redox biologie
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation and In vitro Culture of Bone Marrow-Derived Macrophages for the Study of NO-Redox Biology
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Isolatie en in vitro kweek van beenmerg-afgeleide macrofagen voor de studie van NO-redox biologie

Nederlands

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7,552 Views

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06:34 min

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May 31, 2022

DOI:

10.3791/62834-v

DOI:
10.3791/62834-v

•

06:34 min
May 31, 2022

•

58 Views
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1BHF Centre of Research Excellence, Division of Cardiovascular Medicine, Radcliffe Department of Medicine, John Radcliffe Hospital,University of Oxford, 2Wellcome Centre for Human Genetics,University of Oxford

Summary

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Dit protocol is vastgesteld voor het kweken van tetrahydrobiopterine (BH4) - en induceerbare stikstofmonoxidesynthase (iNOS) - deficiënte primaire murine macrofagen om NO-redox biologie te bestuderen. De studie richt zich op het verminderen van potentiële besmetting van BH4 en andere artefacten gevonden in traditionele isolatie- en kweekmethoden die experimentele uitkomsten en interpretatie van resultaten kunnen verstoren.

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Cite this Article

Diotallevi, M., Nicol, T., Ayaz, F., Bailey, J., Shaw, A., McNeill, E., Davies, B., Channon, K. M., Crabtree, M. J. Isolation and In vitro Culture of Bone Marrow-Derived Macrophages for the Study of NO-Redox Biology. J. Vis. Exp. (183), e62834, doi:10.3791/62834 (2022).

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