Journal
/
Biochemie
/
Polysome Profiling without Gradient Makers or Fractionation Systems
JoVE Journal
Biochemie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Biochemie
Polysome Profiling without Gradient Makers or Fractionation Systems

Polysome Profiling without Gradient Makers or Fractionation Systems

4,319 Views

•

05:56 min

•

June 01, 2021

DOI:

10.3791/62680-v

DOI:
10.3791/62680-v

•

05:56 min
June 01, 2021

•

40 Views
,
1Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Department of Health and Human Services,National Institutes of Health

Summary

Automatically generated

This protocol describes how to generate a polysome profile without using automated gradient makers or gradient fractionation systems.

Explore More Videos

Polysome ProfilingGradient-freeSucrose GradientRibosome ActivityYeast Cell LysisRNA ExtractionRNA Quantification

Read Article

Cite this Article

Sobhany, M., Stanley, R. E. Polysome Profiling without Gradient Makers or Fractionation Systems. J. Vis. Exp. (172), e62680, doi:10.3791/62680 (2021).

Download .ris file

Copy

Share Video

.

Copy