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Polysome Profiling without Gradient Makers or Fractionation Systems
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JoVE Journal Bioquímica
Polysome Profiling without Gradient Makers or Fractionation Systems

Polysome Profiling without Gradient Makers or Fractionation Systems

DOI:
10.3791/62680-v

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05:56 min

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June 01, 2021

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,
1Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Department of Health and Human Services,National Institutes of Health

Capítulos

  • 00:04Introduction
  • 00:39Preparation of 7 to 47% Sucrose Gradients
  • 02:14Preparation of Yeast Cell Extracts
  • 03:21Centrifugation of Gradients
  • 03:49Fraction and Data Collection
  • 04:36Results: Polysome Profiling Without Gradient Makers or Fractionation Systems
  • 05:19Conclusion

Summary

Tadução automática

Tadução automática

This protocol describes how to generate a polysome profile without using automated gradient makers or gradient fractionation systems.

Tags

Polysome ProfilingGradient-freeSucrose GradientRibosome ActivityYeast Cell LysisRNA ExtractionRNA Quantification

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