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Modelo Biomimético Tridimensional In Vitro de Neuroblastoma Utilizando Arcabouços à Base de Colágeno
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July 09, 2021
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1Cancer Bioengineering Group, Department of Anatomy and Regenerative Medicine,RCSI University of Medicine and Health Sciences, Dublin, Ireland, 2School of Pharmacy and Biomolecular Sciences,RCSI University of Medicine and Health Sciences, Dublin, Ireland, 3Tissue Engineering Research Group, Department of Anatomy and Regenerative Medicine,RCSI University of Medicine and Health Sciences, Dublin, Ireland, 4National Children’s Research Centre,Our Lady’s Children’s Hospital Crumlin, Dublin, Ireland, 5Trinity Centre for Bioengineering,Trinity College Dublin, Dublin, Ireland, 6Advanced Materials and Bioengineering Research Centre (AMBER),RCSI and TCD, Dublin, Ireland
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Este artigo lista as etapas necessárias para semear linhagens celulares de neuroblastoma em arcabouços tridimensionais à base de colágeno previamente descritos, manter o crescimento celular por um período de tempo pré-determinado e recuperar arcabouços para várias análises de crescimento e comportamento celular e aplicações a jusante, adaptáveis para satisfazer uma variedade de objetivos experimentais.
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Gallagher, C., Murphy, C., Kelly, G., O’Brien, F. J., Piskareva, O. Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds. J. Vis. Exp. (173), e62627, doi:10.3791/62627 (2021).
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