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コラーゲンベースの足場を用いた神経芽細胞腫の3次元 in vitro 生体模倣モデル

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds

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コラーゲンベースの足場を用いた神経芽細胞腫の3次元 in vitro 生体模倣モデル

3,072 Views

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07:48 min

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July 09, 2021

DOI:

10.3791/62627-v

DOI:

10.3791/62627-v

•

07:48 min

July 09, 2021

•

10 Views

Ciara Gallagher*^1,2,3, Catherine Murphy*^1,2,3, Graeme Kelly⁴, Fergal J. O’Brien^3,5,6, Olga Piskareva^1,2,3,6,7

¹Cancer Bioengineering Group, Department of Anatomy and Regenerative Medicine,RCSI University of Medicine and Health Sciences, Dublin, Ireland, ²School of Pharmacy and Biomolecular Sciences,RCSI University of Medicine and Health Sciences, Dublin, Ireland, ³Tissue Engineering Research Group, Department of Anatomy and Regenerative Medicine,RCSI University of Medicine and Health Sciences, Dublin, Ireland, ⁴National Children’s Research Centre,Our Lady’s Children’s Hospital Crumlin, Dublin, Ireland, ⁵Trinity Centre for Bioengineering,Trinity College Dublin, Dublin, Ireland, ⁶Advanced Materials and Bioengineering Research Centre (AMBER),RCSI and TCD, Dublin, Ireland

Summary

この論文では、神経芽腫細胞株を以前に説明した3次元コラーゲンベースの足場に播種し、所定の時間枠で細胞増殖を維持し、さまざまな実験目的を満たすために適応可能な、いくつかの細胞増殖および細胞挙動分析およびダウンストリームアプリケーション用の足場を回収するために必要な手順をリストしています。

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