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Modello biomimetico tridimensionale in vitro di neuroblastoma utilizzando scaffold a base di collagene
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July 09, 2021
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1Cancer Bioengineering Group, Department of Anatomy and Regenerative Medicine,RCSI University of Medicine and Health Sciences, Dublin, Ireland, 2School of Pharmacy and Biomolecular Sciences,RCSI University of Medicine and Health Sciences, Dublin, Ireland, 3Tissue Engineering Research Group, Department of Anatomy and Regenerative Medicine,RCSI University of Medicine and Health Sciences, Dublin, Ireland, 4National Children’s Research Centre,Our Lady’s Children’s Hospital Crumlin, Dublin, Ireland, 5Trinity Centre for Bioengineering,Trinity College Dublin, Dublin, Ireland, 6Advanced Materials and Bioengineering Research Centre (AMBER),RCSI and TCD, Dublin, Ireland
Summary
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Questo documento elenca i passaggi necessari per seminare linee cellulari di neuroblastoma su scaffold tridimensionali a base di collagene precedentemente descritti, mantenere la crescita cellulare per un periodo di tempo predeterminato e recuperare scaffold per diverse analisi della crescita cellulare e del comportamento cellulare e applicazioni a valle, adattabili per soddisfare una serie di obiettivi sperimentali.
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Gallagher, C., Murphy, C., Kelly, G., O’Brien, F. J., Piskareva, O. Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds. J. Vis. Exp. (173), e62627, doi:10.3791/62627 (2021).
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