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Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds
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Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds
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July 09, 2021
DOI:
1Cancer Bioengineering Group, Department of Anatomy and Regenerative Medicine,RCSI University of Medicine and Health Sciences, Dublin, Ireland, 2School of Pharmacy and Biomolecular Sciences,RCSI University of Medicine and Health Sciences, Dublin, Ireland, 3Tissue Engineering Research Group, Department of Anatomy and Regenerative Medicine,RCSI University of Medicine and Health Sciences, Dublin, Ireland, 4National Children’s Research Centre,Our Lady’s Children’s Hospital Crumlin, Dublin, Ireland, 5Trinity Centre for Bioengineering,Trinity College Dublin, Dublin, Ireland, 6Advanced Materials and Bioengineering Research Centre (AMBER),RCSI and TCD, Dublin, Ireland
Summary
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This paper lists the steps required to seed neuroblastoma cell lines on previously described three-dimensional collagen-based scaffolds, maintain cell growth for a predetermined timeframe, and retrieve scaffolds for several cell growth and cell behavior analyses and downstream applications, adaptable to satisfy a range of experimental aims.
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Gallagher, C., Murphy, C., Kelly, G., O’Brien, F. J., Piskareva, O. Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds. J. Vis. Exp. (173), e62627, doi:10.3791/62627 (2021).
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