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数字液滴PCR检测转基因按蚊种群中的Indels突变

JoVE Journal
Genetics

This content is Free Access.

JoVE Journal Genetics

Digital-Droplet PCR to Detect Indels Mutations in Genetically Modified Anopheline Mosquito Populations

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数字液滴PCR检测转基因按蚊种群中的Indels突变

3,004 Views

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05:51 min

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June 28, 2021

DOI:

10.3791/62607-v

DOI:

10.3791/62607-v

•

05:51 min

June 28, 2021

•

14 Views

Rebeca Carballar-Lejarazú¹, Thai Binh Pham², Adam Kelsey¹, Taylor Tushar¹, Anthony A. James^1,2

¹Department of Microbiology & Molecular Genetics,University of California, Irvine, ²Department of Molecular Biology & Biochemistry,University of California, Irvine

Summary

该协议提供了从DNA提取到数字液滴PCR（ddPCR）实验设置的步骤，包括分析gRNA诱导的Cas9切割和DNA修复后靶位点的非同源末端连接（NHEJ）事件。该方法的其他用途包括多态性检测和基因编辑变异验证等应用。

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