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Digital-Droplet PCR to Detect Indels Mutations in Genetically Modified Anopheline Mosquito Populations

Journal JoVE
Génétique

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Journal JoVE Génétique

Digital-Droplet PCR to Detect Indels Mutations in Genetically Modified Anopheline Mosquito Populations

Digital-Droplet PCR to Detect Indels Mutations in Genetically Modified Anopheline Mosquito Populations

2,945 Views

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05:51 min

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June 28, 2021

DOI:

10.3791/62607-v

DOI:

10.3791/62607-v

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05:51 min

June 28, 2021

•

3 Views

Rebeca Carballar-Lejarazú, Thai Binh Pham, Adam Kelsey, Taylor Tushar, Anthony A. James²

¹Department of Microbiology & Molecular Genetics,University of California, Irvine, ²Department of Molecular Biology & Biochemistry,University of California, Irvine

Tags

Digital Droplet PCR DdPCR NHEJ Analysis Indel Mutations Gene-edited Mosquito Genetic Variation High Throughput Fast Turnaround Field Analysis Genetically Modified Organisms

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