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Digital-Droplet PCR to Detect Indels Mutations in Genetically Modified Anopheline Mosquito Populations
JoVE Journal
Genetik
This content is Free Access.
JoVE Journal Genetik
Digital-Droplet PCR to Detect Indels Mutations in Genetically Modified Anopheline Mosquito Populations

Digital-Droplet PCR to Detect Indels Mutations in Genetically Modified Anopheline Mosquito Populations

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05:51 min

June 28, 2021

DOI:

05:51 min
June 28, 2021

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Summary

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This protocol provides the steps from DNA extraction to experimental set-up for digital droplet PCR (ddPCR), including analysis for the identification and quantification of non-homologous end-joining (NHEJ) events at target sites following gRNA-induced Cas9 cleavage and DNA repair. Other uses of this method include applications such as polymorphism detection and gene-editing variant verification.

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