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Fabricação simples de coluna capilar de alto desempenho com a abordagem flashpack
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Fabricação simples de coluna capilar de alto desempenho com a abordagem flashpack

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3,574 Views

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13:36 min

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December 04, 2021

DOI:

10.3791/62522-v

DOI:
10.3791/62522-v

•

13:36 min
December 04, 2021

•

8 Views
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1Laboratory of Bioinformatics Methods in Combinatorial Chemistry and Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences, 2Department of Molecular Neuroimmune Signalling, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences

Summary

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Aqui apresentamos um protocolo para o procedimento otimizado de embalagem de colunas capilares FlashPack. A aplicação de um protocolo otimizado para uma configuração comum de bomba de pressão de 100 barras permite a embalagem e fabricação 10 vezes mais rápidas de colunas capilares de alto desempenho.

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Kovalchuk, S. I., Ziganshin, R., Shelukhina, I. Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach. J. Vis. Exp. (178), e62522, doi:10.3791/62522 (2021).

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