Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Simple In-House Ultra-High Performance Capillary…
JoVE Journal
Biochemistry
Author Produced

Esto contenido es Free Access.

JoVE Journal Biochemistry
Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
 

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Article DOI: 10.3791/62522-v • 13:36 min • December 4th, 2021
December 4th, 2021 •
1, 1, 2
1Laboratory of Bioinformatics Methods in Combinatorial Chemistry and Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 2Department of Molecular Neuroimmune Signalling, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Capítulos

Resumen
Automatic Translation

Here we present a protocol for the optimized FlashPack capillary column packing procedure. Application of an optimized protocol to a common 100-bar pressure bomb setup allows 10-times faster packing and manufacturing of long ultra-high performance capillary columns.

Tags

In-house Ultra-high Performance Capillary Column FlashPack Approach LC-MS Proteomics Analysis Mass Spectrometry Liquid Chromatography Peptide Separation Internal Diameter Sorbent Column Size Customization Packing Procedure Sorbent Slurry Concentration Pressure Bomb Packing Setup Sorbent Clusters
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter