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使用FlashPack方法进行简单的内部超高性能毛细管柱制造
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
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使用FlashPack方法进行简单的内部超高性能毛细管柱制造

DOI:
10.3791/62522-v

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13:36 min

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December 04, 2021

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, ,
1Laboratory of Bioinformatics Methods in Combinatorial Chemistry and Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences, 2Department of Molecular Neuroimmune Signalling, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences

Chapters

  • 00:00Introduction
  • 01:34Protocol Introduction
  • 02:06Stage 1:Packing Station Assembly
  • 02:26Stage 2: Capillary Preparation
  • 02:59Stage 3: Sorbent Slurry Preparation
  • 04:45Stage 4: Capillary Packing in a Pressure Bomb
  • 09:09Stage 5: Packing in the HPLC System
  • 11:35Representative Results
  • 12:59Conclusions

Summary

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在这里，我们提出了一个优化的FlashPack毛细管柱包装程序的协议。将优化的实验方案应用于常见的 100 bar 压力弹设置，可将长超高性能毛细管柱的包装和制造速度提高 10 倍。

Tags

In-house Capillary Column ManufacturingFlashPack ApproachUltra-high Performance Capillary Liquid ChromatographyLC-MS ProteomicsPeptide SeparationCapillary ColumnsSorbent PackingHigh Sorbent Slurry ConcentrationPressure Bomb PackingMagnet BarPacking RateUltra-high Performance SorbentsLong ColumnsColumn Preparation ProcedurePacking StationNitrogen Gas TankPressure BombMagnetic StirrerPEEK Capillary

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