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Immunology and Infection

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HiBiTベースバイオレポーターを用いたSARS-CoV-2受容体結合ドメイン抗体の検出

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

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HiBiTベースバイオレポーターを用いたSARS-CoV-2受容体結合ドメイン抗体の検出

2,932 Views

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07:44 min

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August 12, 2021

DOI:

10.3791/62488-v

DOI:

10.3791/62488-v

•

07:44 min

August 12, 2021

•

1 Views

Reza Rezaei^1,2, Abera Surendran^1,2, Ragunath Singaravelu^1,2, Taylor R. Jamieson^1,2, Parisa Taklifi³, Joanna Poutou^1,2, Taha Azad^1,2, Carolina S. Ilkow^1,2

¹Ottawa Hospital Research Institute, ²Department of Biochemistry, Microbiology and Immunology,University of Ottawa, ³Department of Biotechnology,University of Tehran

Summary

概説されたプロトコルは、HiBiT受容体結合ドメインタンパク質複合体を産生するための手順と、SARS-CoV-2抗体の迅速かつ感度的な検出のためのその応用について説明する。

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