Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

Reza Rezaei; Abera Surendran; Ragunath Singaravelu; Taylor R. Jamieson; Parisa Taklifi; Joanna Poutou; Taha Azad; Carolina S. Ilkow

doi:10.3791/62488

Journal

/

면역학 및 감염병학

/

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

JoVE 신문
면역학 및 감염병학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 면역학 및 감염병학

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

2,932 Views

•

07:44 min

•

August 12, 2021

DOI:

10.3791/62488-v

DOI:

10.3791/62488-v

•

07:44 min

August 12, 2021

•

1 Views

Reza Rezaei^1,2, Abera Surendran^1,2, Ragunath Singaravelu^1,2, Taylor R. Jamieson^1,2, Parisa Taklifi³, Joanna Poutou^1,2, Taha Azad^1,2, Carolina S. Ilkow^1,2

¹Ottawa Hospital Research Institute, ²Department of Biochemistry, Microbiology and Immunology,University of Ottawa, ³Department of Biotechnology,University of Tehran

Summary

The outlined protocol describes the procedure for producing the HiBiT-receptor-binding domain protein complex and its application for fast and sensitive detection of SARS-CoV-2 antibodies.

Explore More Videos

SARS-CoV-2 Receptor-binding Domain Antibody Detection HiBiT-based Bioreporter COVID-19 Research High-throughput Assay Cell Culture Transfection Passive Lysis Buffer

Read Article

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

Summary

Automatically generated

Explore More Videos