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Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter
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Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

2,932 Views

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07:44 min

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August 12, 2021

DOI:

10.3791/62488-v

DOI:
10.3791/62488-v

•

07:44 min
August 12, 2021

•

1 Views
, , , , , , ,
1Ottawa Hospital Research Institute, 2Department of Biochemistry, Microbiology and Immunology,University of Ottawa, 3Department of Biotechnology,University of Tehran

Summary

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The outlined protocol describes the procedure for producing the HiBiT-receptor-binding domain protein complex and its application for fast and sensitive detection of SARS-CoV-2 antibodies.

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SARS-CoV-2Receptor-binding DomainAntibody DetectionHiBiT-based BioreporterCOVID-19 ResearchHigh-throughput AssayCell CultureTransfectionPassive Lysis Buffer

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Cite this Article

Rezaei, R., Surendran, A., Singaravelu, R., Jamieson, T. R., Taklifi, P., Poutou, J., Azad, T., Ilkow, C. S. Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter. J. Vis. Exp. (174), e62488, doi:10.3791/62488 (2021).

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