Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

Reza Rezaei; Abera Surendran; Ragunath Singaravelu; Taylor R. Jamieson; Parisa Taklifi; Joanna Poutou; Taha Azad; Carolina S. Ilkow

doi:10.3791/62488

Journal

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Immunologie et infection

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Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

Journal JoVE
Immunologie et infection

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Journal JoVE Immunologie et infection

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

2,932 Views

•

07:44 min

•

August 12, 2021

DOI:

10.3791/62488-v

DOI:

10.3791/62488-v

•

07:44 min

August 12, 2021

•

1 Views

Reza Rezaei^1,2, Abera Surendran^1,2, Ragunath Singaravelu^1,2, Taylor R. Jamieson^1,2, Parisa Taklifi³, Joanna Poutou^1,2, Taha Azad^1,2, Carolina S. Ilkow^1,2

¹Ottawa Hospital Research Institute, ²Department of Biochemistry, Microbiology and Immunology,University of Ottawa, ³Department of Biotechnology,University of Tehran

Summary

The outlined protocol describes the procedure for producing the HiBiT-receptor-binding domain protein complex and its application for fast and sensitive detection of SARS-CoV-2 antibodies.

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Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

Summary

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