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基于肽矩阵的 HBV 特异性 CD4 T 细胞响应和 HLA-DR 受限 CD4 T 细胞表位的识别分析

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Immunology and Infection

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JoVE Journal Immunology and Infection

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix

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基于肽矩阵的 HBV 特异性 CD4 T 细胞响应和 HLA-DR 受限 CD4 T 细胞表位的识别分析

DOI:

10.3791/62387-v

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10:37 min

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October 20, 2021

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Jianmei Xiao², Xing Wan², Haoliang Wang², Guohong Deng²

¹Department of Infectious Diseases,Southwest Hospital, Third Military Medical University (Army Medial University), ²Chongqing Key Laboratory for Research of Infectious Diseases

Chapters

00:04Introduction
00:51Isolation of PBMCs and In Vitro Expansion Using an HBV Peptide Matrix
03:55Analysis of HBV-specific CD4 T-Cell Responses by Intracellular Flow Cytometry
05:54Identification of HBV-specific HLA-DR Restricted CD4 T-Cell Epitopes
08:07Results: Identification of HBV-specific HLA-DR Restricted CD4 T-Cell Epitopes
10:06Conclusion

Summary

Automatic Translation

根据乙型肝炎病毒（HBV）衍生肽基质，可在识别乙肝病毒特异性CD4 T细胞表位的同时，对乙肝病毒特异性CD4 T细胞反应进行评估。

Tags

Keywords: HBV-specific CD4 T-cell Responses HLA-DR-restricted CD4 T-cell Epitopes Chronic HBV Infection PBMC Isolation Peptide Matrix CD4 T-cell Response Analysis HBV-derived Peptide Pools IL-2 IL-7 96-well Plates

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