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The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo
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Immunology and Infection
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JoVE Journal Immunology and Infection
The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo

The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo

DOI:
10.3791/51627-v

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12:09 min

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June 19, 2014

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, , ,
1Department of Immunology, John Curtin School of Medical Research,Australian National University

Chapters

  • 00:05Title
  • 01:39Fluorescent Target Array Preparation: CTV and CFSE Labeling
  • 04:54FTA Preparation: Peptide Pulsing and Cell Washing
  • 07:50FTA Preparation: CPD Cell Labeling
  • 09:43FTA Assay in Response to VV/HIV Epitopes
  • 11:45Conclusion

Summary

Automatic Translation

Automatic Translation

The ability to monitor T cell responses in detail in vivo is important for the development of our understanding of the immune response. Here we describe the use of fluorescent target arrays (FTAs) in an in vivo T cell assay that assesses >250 parameters simultaneously by flow cytometry.

Tags

Fluorescent Target ArrayFTACFSECTVCPDMHC Class IMHC Class IICD8+ T CellsCD4+ T CellsT Cell ResponseIn VivoFlow CytometryAntigen EpitopesRecombinant Pox Virus Vaccine

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