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Análise de transcriptome de alto rendimento para investigar interações host-pathogen
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Immunology and Infection
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JoVE Journal Immunology and Infection
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Análise de transcriptome de alto rendimento para investigar interações host-pathogen

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3,868 Views

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14:58 min

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March 05, 2022

DOI:

10.3791/62324-v

DOI:
10.3791/62324-v

•

14:58 min
March 05, 2022

•

3 Views
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1Laboratory of Pathology of Infectious Diseases, Department of Pathology, Medical School,University of São Paulo, 2Scientific Platform Pasteur USP, 3Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences,University of São Paulo, 4Hospital Israelita Albert Einstein

Summary

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O protocolo aqui apresentado descreve um pipeline completo para analisar dados de transcriptome de sequenciamento de RNA desde leituras brutas até análises funcionais, incluindo etapas de controle de qualidade e pré-processamento para abordagens analíticas estatísticas avançadas.

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Cite this Article

Aquime Gonçalves, A. N., Escolano Maso, V., Maia Santos de Castro, Í., Pereira Vasconcelos, A., Tomio Ogava, R. L., I Nakaya, H. High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions. J. Vis. Exp. (181), e62324, doi:10.3791/62324 (2022).

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