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Analisi del trascrittoma ad alto rendimento per indagare le interazioni ospite-patogeno
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Immunology and Infection
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JoVE Journal Immunology and Infection
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
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Analisi del trascrittoma ad alto rendimento per indagare le interazioni ospite-patogeno

italiano

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3,866 Views

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14:58 min

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March 05, 2022

DOI:

10.3791/62324-v

DOI:
10.3791/62324-v

•

14:58 min
March 05, 2022

•

3 Views
, , , , ,
1Laboratory of Pathology of Infectious Diseases, Department of Pathology, Medical School,University of São Paulo, 2Scientific Platform Pasteur USP, 3Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences,University of São Paulo, 4Hospital Israelita Albert Einstein

Summary

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Il protocollo qui presentato descrive una pipeline completa per analizzare i dati del trascrittoma di sequenziamento dell'RNA dalle letture grezze all'analisi funzionale, compresi i passaggi di controllo della qualità e pre-elaborazione per approcci analitici statistici avanzati.

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Cite this Article

Aquime Gonçalves, A. N., Escolano Maso, V., Maia Santos de Castro, Í., Pereira Vasconcelos, A., Tomio Ogava, R. L., I Nakaya, H. High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions. J. Vis. Exp. (181), e62324, doi:10.3791/62324 (2022).

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