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Análisis de transcriptoma de alto rendimiento para investigar las interacciones huésped-patógeno
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Immunology and Infection
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JoVE Journal Immunology and Infection
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
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Análisis de transcriptoma de alto rendimiento para investigar las interacciones huésped-patógeno

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3,868 Views

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14:58 min

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March 05, 2022

DOI:

10.3791/62324-v

DOI:
10.3791/62324-v

•

14:58 min
March 05, 2022

•

3 Views
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1Laboratory of Pathology of Infectious Diseases, Department of Pathology, Medical School,University of São Paulo, 2Scientific Platform Pasteur USP, 3Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences,University of São Paulo, 4Hospital Israelita Albert Einstein

Summary

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El protocolo presentado aquí describe una tubería completa para analizar los datos del transcriptoma de secuenciación de ARN desde lecturas sin procesar hasta análisis funcionales, incluidos los pasos de control de calidad y preprocesamiento para enfoques analíticos estadísticos avanzados.

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Cite this Article

Aquime Gonçalves, A. N., Escolano Maso, V., Maia Santos de Castro, Í., Pereira Vasconcelos, A., Tomio Ogava, R. L., I Nakaya, H. High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions. J. Vis. Exp. (181), e62324, doi:10.3791/62324 (2022).

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