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Transcriptoomanalyse met hoge doorvoer voor het onderzoeken van gastheer-pathogeeninteracties
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Immunology and Infection
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JoVE Journal Immunology and Infection
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
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Transcriptoomanalyse met hoge doorvoer voor het onderzoeken van gastheer-pathogeeninteracties

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3,868 Views

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14:58 min

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March 05, 2022

DOI:

10.3791/62324-v

DOI:
10.3791/62324-v

•

14:58 min
March 05, 2022

•

3 Views
, , , , ,
1Laboratory of Pathology of Infectious Diseases, Department of Pathology, Medical School,University of São Paulo, 2Scientific Platform Pasteur USP, 3Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences,University of São Paulo, 4Hospital Israelita Albert Einstein

Summary

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Het hier gepresenteerde protocol beschrijft een complete pijplijn om RNA-sequencing transcriptoomgegevens te analyseren, van ruwe reads tot functionele analyse, inclusief kwaliteitscontrole en voorbewerkingsstappen tot geavanceerde statistische analytische benaderingen.

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Cite this Article

Aquime Gonçalves, A. N., Escolano Maso, V., Maia Santos de Castro, Í., Pereira Vasconcelos, A., Tomio Ogava, R. L., I Nakaya, H. High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions. J. Vis. Exp. (181), e62324, doi:10.3791/62324 (2022).

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