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極低温保存細胞からの微視的データ収集
JoVE Journal
Biology
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JoVE Journal Biology
Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
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極低温保存細胞からの微視的データ収集

日本語

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3,860 Views

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11:55 min

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May 28, 2021

DOI:

10.3791/62274-v

DOI:
10.3791/62274-v

•

11:55 min
May 28, 2021

•

4 Views
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1Harwell Science and Innovation Campus,Beamline B24, Diamond Light Source, 2Division of Virology, Department of Pathology,University of Cambridge, 3ALBA Synchrotron,Beamline 09 – MISTRAL, 4Micron Advanced Imaging Consortium, Department of Biochemistry,University of Oxford

Summary

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このプロトコルは、クライオ構造の照明顕微鏡を用いて、生物学的に凍結保存されたサンプルを画像化する方法を示す。U2OS細胞の細胞骨格をイメージングして方法論を実証する。

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Cite this Article

Vyas, N., Perry, N., Okolo, C. A., Kounatidis, I., Fish, T. M., Nahas, K. L., Jadhav, A., Koronfel, M. A., Groen, J., Pereiro, E., Dobbie, I. M., Harkiolaki, M. Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells. J. Vis. Exp. (171), e62274, doi:10.3791/62274 (2021).

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