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Kryostrukturierte Beleuchtung Mikroskopische Datenerfassung von kryogen konservierten Zellen
JoVE Journal
Biology
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JoVE Journal Biology
Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
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Kryostrukturierte Beleuchtung Mikroskopische Datenerfassung von kryogen konservierten Zellen

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3,860 Views

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11:55 min

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May 28, 2021

DOI:

10.3791/62274-v

DOI:
10.3791/62274-v

•

11:55 min
May 28, 2021

•

4 Views
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1Harwell Science and Innovation Campus,Beamline B24, Diamond Light Source, 2Division of Virology, Department of Pathology,University of Cambridge, 3ALBA Synchrotron,Beamline 09 – MISTRAL, 4Micron Advanced Imaging Consortium, Department of Biochemistry,University of Oxford

Summary

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Dieses Protokoll zeigt, wie biologische kryokonservierte Proben mit kryostrukturierter Beleuchtungsmikroskopie abgebildet werden können. Wir demonstrieren die Methodik, indem wir das Zytoskelett von U2OS-Zellen abbilden.

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Cite this Article

Vyas, N., Perry, N., Okolo, C. A., Kounatidis, I., Fish, T. M., Nahas, K. L., Jadhav, A., Koronfel, M. A., Groen, J., Pereiro, E., Dobbie, I. M., Harkiolaki, M. Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells. J. Vis. Exp. (171), e62274, doi:10.3791/62274 (2021).

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