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Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
JoVE Journal
Biologia
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JoVE Journal Biologia
Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

3,854 Views

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11:55 min

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May 28, 2021

DOI:

10.3791/62274-v

DOI:
10.3791/62274-v

•

11:55 min
May 28, 2021

•

4 Views
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1Harwell Science and Innovation Campus,Beamline B24, Diamond Light Source, 2Division of Virology, Department of Pathology,University of Cambridge, 3ALBA Synchrotron,Beamline 09 – MISTRAL, 4Micron Advanced Imaging Consortium, Department of Biochemistry,University of Oxford

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This protocol demonstrates how to image biological cryo-preserved samples using cryo-structured illumination microscopy. We demonstrate the methodology by imaging the cytoskeleton of U2OS cells.

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Vyas, N., Perry, N., Okolo, C. A., Kounatidis, I., Fish, T. M., Nahas, K. L., Jadhav, A., Koronfel, M. A., Groen, J., Pereiro, E., Dobbie, I. M., Harkiolaki, M. Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells. J. Vis. Exp. (171), e62274, doi:10.3791/62274 (2021).

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