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Cryo-gestructureerde verlichting Microscopische gegevensverzameling van cryogene geconserveerde cellen
JoVE Journal
Biology
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JoVE Journal Biology
Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
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Cryo-gestructureerde verlichting Microscopische gegevensverzameling van cryogene geconserveerde cellen

Nederlands

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3,860 Views

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11:55 min

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May 28, 2021

DOI:

10.3791/62274-v

DOI:
10.3791/62274-v

•

11:55 min
May 28, 2021

•

4 Views
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1Harwell Science and Innovation Campus,Beamline B24, Diamond Light Source, 2Division of Virology, Department of Pathology,University of Cambridge, 3ALBA Synchrotron,Beamline 09 – MISTRAL, 4Micron Advanced Imaging Consortium, Department of Biochemistry,University of Oxford

Summary

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Dit protocol demonstreert hoe biologische cryo-geconserveerde monsters kunnen worden afgebeeld met behulp van cryo-gestructureerde verlichtingsmicroscopie. We demonstreren de methodologie door het cytoskelet van U2OS-cellen in beeld te brengen.

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Vyas, N., Perry, N., Okolo, C. A., Kounatidis, I., Fish, T. M., Nahas, K. L., Jadhav, A., Koronfel, M. A., Groen, J., Pereiro, E., Dobbie, I. M., Harkiolaki, M. Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells. J. Vis. Exp. (171), e62274, doi:10.3791/62274 (2021).

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