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Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions
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Immunology and Infection
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JoVE Journal Immunology and Infection
Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions

Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions

English

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5,424 Views

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07:35 min

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July 10, 2021

DOI:

10.3791/62219-v

DOI:
10.3791/62219-v

•

07:35 min
July 10, 2021

•

24 Views
, ,
1Laboratory of Cell and Molecular Biology of Trypanosomatids, Department of Cellular and Molecular Biology and Pathogenic Bioagents, Ribeirao Preto Medical School,University of Sao Paulo, Ribeirao Preto, SP, Brazil

Summary

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Here, the protocol describes how to perform double labeling immunofluorescence using primary antibodies raised in the same species to study host-pathogen interactions. Also, it can include the third antibody from a different host in this protocol. This approach can be made in any cell type and pathogens.

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Cite this Article

Gachet-Castro, C., Trajano-Silva, L. A. M., Baqui, M. M. A. Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions. J. Vis. Exp. (173), e62219, doi:10.3791/62219 (2021).

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