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Journal / Immunology and Infection / Double Labeling Immunofluorescence using…
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Immunology and Infection
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JoVE Journal Immunology and Infection
Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions
 

Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions

Article DOI: 10.3791/62219-v • 07:35 min • July 10th, 2021
July 10th, 2021 •
*1, *1, 1
1Laboratory of Cell and Molecular Biology of Trypanosomatids, Department of Cellular and Molecular Biology and Pathogenic Bioagents, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, SP, Brazil
* These authors contributed equally

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Summary
Automatic Translation

Here, the protocol describes how to perform double labeling immunofluorescence using primary antibodies raised in the same species to study host-pathogen interactions. Also, it can include the third antibody from a different host in this protocol. This approach can be made in any cell type and pathogens.

Tags

Double Labeling Immunofluorescence Antibodies Same Species Host-pathogen Interactions Protocol Cell Structures Proteins Parasites Polyclonal Antibodies Monoclonal Antibodies Source Of Antibodies Infected Host Cells Free Living Organisms Blocking Step Trypanosoma Cruzi
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