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Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions
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Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions
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07:35 min
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July 10, 2021
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Summary
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Here, the protocol describes how to perform double labeling immunofluorescence using primary antibodies raised in the same species to study host-pathogen interactions. Also, it can include the third antibody from a different host in this protocol. This approach can be made in any cell type and pathogens.
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Gachet-Castro, C., Trajano-Silva, L. A. M., Baqui, M. M. A. Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions. J. Vis. Exp. (173), e62219, doi:10.3791/62219 (2021).
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