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Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions
Bölümler
- 00:04Introduction
- 01:21Parasite Cultures
- 02:00Control Immunofluorescence Protocol
- 03:23Double Labeling Immunofluorescence ProtocolUsing Monoclonal and Polyclonal Antibodies Raised in the Same Host
- 04:18Triple Labeling Immunofluorescence Protocol with the Addition of the Third Polyclonal Antibody from Different Host Species
- 05:16Confocal Imaging Acquisition
- 05:39Results: Host-pathogen Interactions Studies Using Double Labeling Immunofluorescence
- 06:51Conclusion
Here, the protocol describes how to perform double labeling immunofluorescence using primary antibodies raised in the same species to study host-pathogen interactions. Also, it can include the third antibody from a different host in this protocol. This approach can be made in any cell type and pathogens.
