ジャーナル
/
免疫学と感染
/
Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies
JoVE Journal
免疫学と感染
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies

Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies

DOI:
10.3791/62214-v

•

08:37 min

•

February 15, 2021

•

, , ,
1Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases,IRCCS San Raffaele Scientific Institute, 2Vita-Salute San Raffaele University

章

  • 00:04Introduction
  • 00:39Spleen Processing
  • 01:29T Cell Enrichment
  • 03:27iNKT Cell Enrichment
  • 05:21iNKT Cell Activation and Expansion
  • 06:35Results: Enrichment, Expansion, and Characterization of iNKT Cells
  • 07:50Conclusion

概要

自動翻訳

自動翻訳

We describe a rapid and robust protocol to enrich invariant natural killer T (iNKT) cells from mouse spleen and expand them in vitro to suitable numbers for in vitro and in vivo studies.

タグ

Mouse Invariant Natural Killer T CellsPurificationExpansionIn VitroIn VivoCell IsolationCell EnrichmentMACSCell StainingCell SeparationCell Culture

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Retroviral Transduction of T-cell Receptors in Mouse T-cells

Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays

piggyBac Transposon System Modification of Primary Human T Cells

Artificial Antigen Presenting Cell (aAPC) Mediated Activation and Expansion of Natural Killer T Cells

Killer Artificial Antigen Presenting Cells (KaAPC) for Efficient In Vitro Depletion of Human Antigen-specific T Cells

Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells

An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen

Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels

Read Article