We describe a rapid and robust protocol to enrich invariant natural killer T (iNKT) cells from mouse spleen and expand them in vitro to suitable numbers for in vitro and in vivo studies.
Delfanti, G., Perini, A., Zappa, E., Fedeli, M. Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies. J. Vis. Exp. (168), e62214, doi:10.3791/62214 (2021).