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時間分解型クライオ電子顕微鏡のための高速グリッド調製
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Biochemistry
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JoVE Journal Biochemistry
Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy
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時間分解型クライオ電子顕微鏡のための高速グリッド調製

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3,691 Views

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10:05 min

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November 06, 2021

DOI:

10.3791/62199-v

DOI:
10.3791/62199-v

•

10:05 min
November 06, 2021

•

7 Views
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1School of Biomedical Sciences, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology,University of Leeds, 2School of Molecular and Cellular Biology, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology,University of Leeds, 3Department of Physiological Sciences,Eastern Virginia Medical School

Summary

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ここでは、高速グリッド作成用の高速グリッド製造装置の使用と、時間分解実験を行うための迅速な混合と凍結の両方に関する詳細なプロトコルを提供します。

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Cite this Article

Klebl, D. P., Sobott, F., White, H. D., Muench, S. P. Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy. J. Vis. Exp. (177), e62199, doi:10.3791/62199 (2021).

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