È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy
3,691 Views
•
10:05 min
•
November 06, 2021
DOI:
1School of Biomedical Sciences, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology,University of Leeds, 2School of Molecular and Cellular Biology, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology,University of Leeds, 3Department of Physiological Sciences,Eastern Virginia Medical School
Summary
Automatically generated
Here, we provide a detailed protocol for the use of a rapid grid making device for both fast grid-making and for rapid mixing and freezing to conduct time-resolved experiments.
Read Article
Cite this Article
Klebl, D. P., Sobott, F., White, H. D., Muench, S. P. Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy. J. Vis. Exp. (177), e62199, doi:10.3791/62199 (2021).
Copy