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Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy
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10:05 min
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November 06, 2021
DOI:
1School of Biomedical Sciences, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology,University of Leeds, 2School of Molecular and Cellular Biology, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology,University of Leeds, 3Department of Physiological Sciences,Eastern Virginia Medical School
Summary
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Here, we provide a detailed protocol for the use of a rapid grid making device for both fast grid-making and for rapid mixing and freezing to conduct time-resolved experiments.
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Klebl, D. P., Sobott, F., White, H. D., Muench, S. P. Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy. J. Vis. Exp. (177), e62199, doi:10.3791/62199 (2021).
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